Recipe

Tissue fixing buffer

Reagent Amount to add (for 40 mL) Final concentration
Paraformaldehyde (16%) (Electron Microscopy Sciences) 10 mL 25%
DMSO 0.4 mL 1%
Phosphate-buffered saline (PBS) (10X) 4 mL 10%
H2O 25.6 mL 64%
Mix reagents in a chemical fume hood in a 50-mL conical tube. Keep on ice at all times. Prepare fresh before use.
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  1. doi:10.1101/pdb.rec11978 Cold Spring Harb Protoc 2009: pdb.rec11978-
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