MSO liquid medium (pH 5.8)
| Reagent | Amount to add (for 1 L) | Final concentration |
|---|---|---|
| Murashige and Skoog (MS) basal salt mixture with vitamins (Phytotechnology Laboratories) | 4.3 g | 0.43% |
| Sucrose | 30 g | 3% |
| H2O | to 1 L | |
| Adjust pH to 5.8 using 1 M NaOH. Autoclave medium and store at 4°C. Medium is good for up to 6 mo. | ||
