Prehybridization buffer for ISH

Formamide (50% [v/v])

Heparin (50 μg/mL)

Prepare in RNase-free water. Filter-sterilize through a 0.2-μm filter.

Salmon sperm DNA, single-stranded (100 μg/mL)

Denature at 90°C-100°C for 10 min before adding.

SDS (sodium dodecyl sulfate) (1% [w/v])

SSC (5X, pH 4.5)

Tween-20 (0.1% [v/v])

Store at −20°C.