Recipe

MS medium for meristem culture

Murashige and Skoog (MS) medium (Sigma-Aldrich)

3% sucrose

20 mg/L cysteine

0.2 mg/L 1-naphthalene acetic acid (NAA) (Sigma-Aldrich)

0.12 mg/L benzylaminopurine (6-benzylaminopurine, BAP) (Sigma-Aldrich)

0.08 mg/L gibberellic acid A3 (GA3) (Sigma-Aldrich)

80 mg/L adenine sulfate

For solid medium, supplement with 0.6% (w/v) agar.

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  1. doi:10.1101/pdb.rec12013 Cold Spring Harb Protoc 2009: pdb.rec12013-

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