Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Cleavage Stages and Blastocysts

Danielle Hickford; Stephen Frankenberg; Marilyn B. Renfree

doi:10.1101/pdb.prot5339

Protocol

Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Cleavage Stages and Blastocysts

Department of Zoology, The University of Melbourne, Victoria 3010, Australia

↵¹Corresponding author (m.renfree{at}unimelb.edu.au).

INTRODUCTION

This protocol describes a method for whole-mount immunofluorescence staining of early stages of tammar wallaby (Macropus eugenii) development, including cleavage and unilaminar blastocysts. Conceptuses of these stages are surrounded by a shell coat, which must be removed before staining to ensure proper penetration of all solutions.