Recipe

D-R buffer A

10 mM HEPES-KOH (pH 7.9 at 4°C)

10 mM KCl

1.5 mM MgCl2

Immediately before use, add:

0.5 mM DTT (dithiothreitol)

0.5 mM PMSF (phenylmethylsulfonyl fluoride)

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  1. doi:10.1101/pdb.rec12024 Cold Spring Harb Protoc 2009: pdb.rec12024-
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