Recipe

Tris buffer (pH 10)

Reagent Quantity (for 1000 mL) Final concentration
Tris base 1.21 g 10 mM
H2O 1000 mL
Dissolve Tris in H2O. Adjust the pH to 10 using 1 N NaOH. Store at room temperature for 3 mo or at 4°C for up to 6 mo.
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  1. doi:10.1101/pdb.rec12043 Cold Spring Harb Protoc 2009: pdb.rec12043-
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