Recipe

Paraformaldehyde (PFA; 4%)

Reagent Quantity (for 100 mL) Final concentration
Paraformaldehyde (PFA) 4 g 4%
Phosphate-buffered saline for immunohistochemistry (10X), diluted to 1X 100 mL
Slowly dissolve PFA in 1X phosphate-buffered saline over low heat until solution clears. Cool. Use immediately or aliquot and store at −20ºC for up to 1 yr.
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  1. doi:10.1101/pdb.rec12044 Cold Spring Harb Protoc 2009: pdb.rec12044-

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