Recipe

LacZ buffer (LZB)

Reagent Quantity (for 50 mL) Final concentration
Phosphate-buffered saline (PBS) without CaCl2 or MgCl2 (10X) 5 mL 1X
K4Fe(CN)6•3H2O (300 mM) 500 μL 3 mM
K3Fe(CN)6 (300 mM) 500 μL 3 mM
MgCl2 (1 M) 50 μL 1 mM
Tween 20 (100.0%) 25 μL 0.05%
H2O to 50 mL
LZB can be stored for several weeks at 4°C unless contaminated.
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec12051 Cold Spring Harb Protoc 2009: pdb.rec12051-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. February 2026, 2026 (2)
  1. Current Issue