Recipe

MEM-GA

Reagent Quantity (for 50 mL) Final concentration
MOPS (1 M, pH 7.4) 5 mL 0.1 M
NaCl (5 M) 5 mL 0.5 M
EGTA (0.5 M, pH 8.0) 100 μL 1 mM
MgSO4 (1 M) 100 μL 2 mM
Glutaraldehyde (25%) 400 μL 0.2%
Tween 20 (100%) 25 μL 0.05%
H2O to 50 mL
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  1. doi:10.1101/pdb.rec12052 Cold Spring Harb Protoc 2009: pdb.rec12052-
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