Recipe

Calcium- and magnesium-free artificial seawater (CMF-ASW)

Reagent Quantity (for 1 L) Final concentration
NaCl 26.24 g 449 mM
KCl 0.671 g 9 mM
Na2SO4 4.687 g 33 mM
NaHCO3 (0.5 M, filtered) 4.3 mL 2.15 mM
Tris-Cl (1 M, pH 8.0) 10 mL 10 mM
EGTA (0.25 M, pH 8.0) 10 mL 2.5 mM
H2O to 1 L
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  1. doi:10.1101/pdb.rec12053 Cold Spring Harb Protoc 2009: pdb.rec12053-
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