Recipe

TMN (Tris-MgCl2-NaCl buffer)

Reagent Quantity (for 50 mL) Final concentration
NaCl (5 M) 1 mL 100 mM
MgCl2 (1 M) 2.5 mL 50 mM
Tris-Cl (1 M, pH 9.5) 5 mL 100 mM
Tween 20 (100%) 25 μL 0.05% (v/v)
H2O to 50 mL
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  1. doi:10.1101/pdb.rec12056 Cold Spring Harb Protoc 2009: pdb.rec12056-
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