Recipe

PVA (10%) in TMN

Reagent Quantity (for ~40 mL) Final concentration
Polyvinyl alcohol (PVA) (30-70 kDa) 4 g 10% (w/v)
TMN 40 mL
Prepare on the day before staining, and stir overnight. The next day, filter the solution on folded, pre-wet filter paper to remove undissolved PVA particles.
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  1. doi:10.1101/pdb.rec12057 Cold Spring Harb Protoc 2009: pdb.rec12057-
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