Recipe

Prehybridization buffer for Ciona (Pre-HB)

Reagent Quantity (for 50 mL) Final concentration
Formamide (100%) 25.0 mL 50% (v/v)
SSC (20X) 15.0 mL 6X
Tween 20 (100%) 25 μL 0.05% (v/v)
H2O to 50 mL
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  1. doi:10.1101/pdb.rec12058 Cold Spring Harb Protoc 2009: pdb.rec12058-
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