Recipe

Solution A for Ciona

Reagent Quantity (for 10 mL) Final concentration
Tris-Cl (1 M, pH 8.0) 100 μL 10 mM
NaCl (5 M) 1 mL 0.5 M
EDTA (0.5 M) 100 μL 5 mM
Tween 20 (100%) 10 μL 0.1% (v/v)
H2O to 10 mL
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  1. doi:10.1101/pdb.rec12059 Cold Spring Harb Protoc 2009: pdb.rec12059-
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