Table

Table 2. Properties of the most useful optical highlighter FP reporters

Protein (acronym) Ex (nm) Em (nm) EC × 10-3 M-1 cm-1 QY Oligomeric structure Relative brightness (% of EGFP) Reference
Photoactivatable FPs
PA-GFP (NA)a 400 515 20.7 0.13 Monomer 8 Patterson and Lippincott-Schwartz 2002
PA-GFP (G)a 504 517 17.4 0.79 Monomer 41 Patterson and Lippincott-Schwartz 2002
PS-CFP2 (C)a 400 468 43.0 0.20 Monomer 26 Chudakov et al. 2004
PS-CFP2 (G) 490 511 47.0 0.23 Monomer 32 Chudakov et al. 2004
PA-mRFP1 (R)a 578 605 10.0 0.08 Monomer 3 Verkhusha and Sorkin 2005
PA-mCherry1 (NA)a 404 NRa 6.5 NR Monomer NR Subach et al. 2009
PA-mCherry1 (R) 564 595 18.0 0.46 Monomer 20 Subach et al. 2009
Photoconvertible FPs
Kaede (G) 508 518 98.8 0.88 Tetramer 259 Ando et al. 2002
Kaede (R) 572 580 60.4 0.33 Tetramer 59 Ando et al. 2002
Kikume (KikGR; G) 507 517 53.7 0.70 Tetramer 112 Tsutsui et al. 2005
Kikume (KikGR; R) 583 593 35.1 0.65 Tetramer 68 Tsutsui et al. 2005
mKikGR (G) 505 515 49.0 0.69 Monomer 101 Habuchi et al. 2008
mKikGR (R) 580 591 28.0 0.63 Monomer 53 Habuchi et al. 2008
tdEos (G) 506 516 84.0 0.66 P-Monomera 165 Nienhaus et al. 2006
tdEos (R) 569 581 33.0 0.60 P-Monomera 59 Nienhaus et al. 2006
Dendra2 (G) 490 507 45.0 0.50 Monomer 67 Gurskaya et al. 2006
Dendra2 (R) 553 573 35.0 0.55 Monomer 57 Gurskaya et al. 2006
mEosFP2 (G) 506 519 56.0 0.84 Monomer 140 McKinney et al. 2009
mEosFP2 (R) 573 584 46.0 0.66 Monomer 90 McKinney et al. 2009
Photoswitchable FPs
Kindling (R) 580 600 59.0 0.07 Tetramer 12 Chudakov et al. 2003
Dronpa (G) 503 518 95.0 0.85 Monomer 240 Ando et al. 2004
aSignifies a weak dimer.
The peak excitation (Ex) and emission (Em) wavelengths, molar extinction coefficient (EC), quantum yield (QY), relative brightness, and physiologically relevant quaternary structure are listed for both the activated and nonactivated species. The computed brightness values were derived from the product of the molar extinction coefficient and quantum yield, divided by the value for EGFP (NA) native or nonphotoactivated species; (G) green species; (C) cyan species; (R) red species; (NR) not reported; (P-Monomera) pseudo monomer (tandem dimer).
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  1. doi:10.1101/pdb.tab2top63 Cold Spring Harb Protoc 2009: pdb.tab2top63-
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