Recipe

Ligation buffer (10X)

ATP (10 mM)

DTT (dithiothreitol; 100 mM)

MgCl2 (100 mM)

Tris-Cl (300 mM, pH 7.8)

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  1. doi:10.1101/pdb.rec11686 Cold Spring Harb Protoc 2009: pdb.rec11686-
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