Recipe

BCIP-NBT stain

Reagent Quantity (for 20 mL) Final concentration
BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (50 mg/mL) 70 μL 175 μg/mL
NBT (4-nitro blue tetrazolium chloride) (100 mg/mL) 67.5 μL 338 μg/mL
NTMT(L) (pH 9.5) to 20 mL

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  1. doi:10.1101/pdb.rec11715 Cold Spring Harb Protoc 2009: pdb.rec11715-
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