Recipe

Buffer R+

EGTA (0.5 mM)

Glycerol (10%, v/v)

KCl (10 mM)

MgCl2 (1.5 mM)

Potassium HEPES (10 mM, pH 7.6)

Store solution for up to 24 h at 4°C.

Immediately before use, add:

β-glycerophosphate (10 mM)

DTT (dithiothreitol) (1 mM)

Phenylmethylsulfonyl fluoride (0.2 mM)

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  1. doi:10.1101/pdb.rec11755 Cold Spring Harb Protoc 2009: pdb.rec11755-
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