Recipe

PvOH/PEG solution

HEG buffer containing:

Polyethylene glycol (PEG) (5%, m.w. 8,000; Sigma-Aldrich P2139)

Polyvinyl alcohol (PvOH) (5%, m.w. 10,000; Sigma-Aldrich P8136)

Store in aliquots of 0.1-1 mL for up to 2 yr at -20°C.

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  1. doi:10.1101/pdb.rec11758 Cold Spring Harb Protoc 2009: pdb.rec11758-
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