Table 1. Restriction enzymes for inverse PCR and plasmid rescue
| Plasmid rescue | ||||||
| P{PZ} | P{lacW} | P{EP} | ||||
| Inverse PCR | 5′ (left) | 3′ (right) | 5′ (left) | 3′ (right) | 5′ (left) | 3′ (right) |
| Sau3AI | XbaI | N/Aa | EcoRI | BamHI | N/Aa | EcoRI |
| HinP1I | XbaI + SpeI | SacII | BglII | |||
| MspI | XbaI + NheI | PstI | ||||
| XbaI | ||||||
| aPlasmid rescue will only work off the 5′ (left) side of P{PZ} and the 3′ (right) side of P{EP}. | ||||||
