Table 2. Primer combinations for PCR and sequencing

Table

	Rescued end	PCR primers		Annealing temperature	Sequencing primers^a
P-element		Forward	Reverse
P{Z}	5′	Plac4	Plac1	60ºC	Splac2	Sp1
P{Z}	3′	Pry4	Pry1	55ºC	Spep1	Sp3
P{lacW}	5′	Plac4	Plac1	60ºC	Splac2	Sp1
P{lacW}^b	3′	Pry4	Plw3-1	55ºC	Spep1	Sp6(Sau3AI)
						Sp5(HinP1I)
P{EP}	5′	Pwht1	Plac1	60ºC	Sp1	--
P{EP}	3′	Pry4	Pry1	55ºC	Spep1	--

^aWhere two primers are listed, the inverse PCR product can be sequenced from both ends by setting up sequencing reactions with the two different primers.
^bFor the rescue of sequences 3′ of P{lacW}, use the Sp6 primer on templates derived from Sau3AI digests, and Sp5 primer on templates derived from HinP1I digests. The Spep1 primer works on both templates.