Recipe

Modified phosphate-buffered saline (10X)

Reagent Quantity Final concentration
NaCl 81.75 g 1.40 M
K2HPO4 10.7 g 61 mM
KH2PO4 4.7 g 39 mM
Adjust the final volume to 1 L with diethyl pyrocarbonate (DEPC)-treated H2O. Adjust the pH to 7, distribute into aliquots, and autoclave.

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  1. doi:10.1101/pdb.rec11780 Cold Spring Harb Protoc 2009: pdb.rec11780-
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