Recipe

Modified phosphate-buffered saline (1X)

Reagent Quantity Final concentration
NaCl 8.175 g 140 mM
K2HPO4 1.07 g 6.1 mM
KH2PO4 0.47 g 3.9 mM
Adjust the final volume to 1 L with diethyl pyrocarbonate (DEPC)-treated H2O. Adjust the pH to 7, distribute into aliquots, and autoclave.

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  1. doi:10.1101/pdb.rec11781 Cold Spring Harb Protoc 2009: pdb.rec11781-
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