Recipe

Hybridization buffer (HB) for butterfly

Reagent Quantity (for 50 mL) Final concentration
Prehybridization buffer (preHB) 50 mL 1X
Yeast tRNA (Roche)
(20 mg/mL; heat-denatured for 5 min at 80°C) 		250 μL 100 μg/mL
Heparin (25 mg/mL) or Glycine (100 mg/mL) 100 μL (heparin) or 0.5-1 mL (glycine) 50 μg/mL (heparin) or 1-2 mg/mL (glycine)

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  1. doi:10.1101/pdb.rec11787 Cold Spring Harb Protoc 2009: pdb.rec11787-
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