YM agar
| Reagent | Amount to add (for 1 L) | Final concentration | Source |
|---|---|---|---|
| Agar | 20 g | 2% (w/v) | Oxoid LP0011 |
| Glucose | 10 g | 1% (w/v) | Sigma-Aldrich G5767 |
| Yeast extract | 3 g | 0.3% (w/v) | BD 212750 |
| Malt extract | 3 g | 0.3% (w/v) | BD 211220 |
| Peptone | 5 g | 0.5% (w/v) | BD 211677 |
| H2O, ultrapure | to 1 L | MilliQ or Barnstead | |
| Alternatively, ready-made YM broth (BD 271120) can be used at 21 g/L in place of the glucose, extracts, and peptone. | |||
| Suspend the reagents in 1 L of ultrapure H2O. If ready-made YM broth is not being used, adjust the pH to 6.2. Sterilize by autoclaving for 15 min at 121°C. | |||
