Protocol

RNA Interference (RNAi) in Honeybee (Apis mellifera) Embryos

  1. Megan J. Wilson
  1. Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand
  1. 1Corresponding author (peter.dearden{at}otago.ac.nz)

INTRODUCTION

This protocol describes RNA interference (RNAi)-mediated knockdown of a target gene in honeybee embryos. dsRNA is directly injected into freshly laid embryos in order to disrupt the function of a specific gene during honeybee embryogenesis.

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