Recipe

MNase digestion buffer for ChIP

50 mM Tris-Cl (pH 7.6)

1 mM CaCl2

0.2% Triton X-100

5 mM sodium butyrate

1X protease inhibitor cocktail for ChIP

0.5 mM phenylmethylsulfonyl fluoride (PMSF), freshly prepared

Nonidet P-40 (NP-40) can be used in place of Triton X-100.

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  1. doi:10.1101/pdb.rec11852 Cold Spring Harb Protoc 2009: pdb.rec11852-
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