Protocol

In Situ Hybridization of Large Milkweed Bug (Oncopeltus) Tissues

  1. Thomas C. Kaufman1
  1. Department of Biology, Indiana University, Bloomington, IN 47405, USA
  1. ↵1Corresponding author (kaufman{at}indiana.edu)

INTRODUCTION

In this protocol, double-label in situ hybridization of large milkweed bug (Oncopeltus) embryos is performed using labeled probes. Dual antibody staining is followed by detection with two different chromagens.

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  1. doi:10.1101/pdb.prot5262 Cold Spring Harb Protoc 2009: pdb.prot5262-
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