Recipe

Tribolium injection buffer (10X)

Reagent Quantity (for 50 mL) Final 10X concentration
5 M NaCl 140 μL 14 mM
10 mM Na2HPO4 3.5 mL 0.7 mM
10 mM KH2PO4 1.5 mL 0.3 mM
100 mM KCl 20 mL 40 mM
H2O to 50 mL
Autoclave and store at −20°C.
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  1. doi:10.1101/pdb.rec11872 Cold Spring Harb Protoc 2009: pdb.rec11872-
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