Recipe

Alkaline phosphatase reaction buffer (APRB)

Reagent Quantity Final concentration
1M Tris-HCl (pH 9.5) 1 mL 100 mM
1M MgCl2 500 μL 50 mM
5M NaCl 200 μL 100 mM
Tween 20 20 μL 0.2%
H2O 8.28 mL
Prepare just before use.
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  1. doi:10.1101/pdb.rec11874 Cold Spring Harb Protoc 2009: pdb.rec11874-
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