Recipe

Hyb A for Tribolium

Reagent Quantity Final concentration
Hyb B for Tribolium 400 mL
Yeast RNA (20 mg/mL) 2 mL 100 μg/mL
Sonicated salmon sperm DNA (10 mg/mL)a 8 mL 200 μg/mL
Heparin (50 mg/mL) 400 μL 50 μg/mL
aBoil the salmon sperm DNA for 10 min and cool on ice for 3 min before adding. Store 16-mL aliquots at −20°C.
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  1. doi:10.1101/pdb.rec11885 Cold Spring Harb Protoc 2009: pdb.rec11885-
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