Recipe

PBS (10X) for Tribolium

Reagent Quantity Final concentration
NaCl 80 g 1.37 M
KCl 2 g 27 mM
KH2PO4 2 g 15 mM
Na2HPO4 11.5 g 80 mM
H2O to 1 L
Adjust pH to 7.4 using NaOH. Autoclave and store at room temperature.
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  1. doi:10.1101/pdb.rec11888 Cold Spring Harb Protoc 2009: pdb.rec11888-
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