Recipe

PBT for Tribolium

Reagent Quantity Final concentration
PBS (10X) for Tribolium 100 mL 1X
20% Tween 20 5 mL 0.1%
H2O 900 mL
Autoclave and keep at room temperature. Use 100-mL aliquots for each staining procedure to avoid contamination of the solution.
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  1. doi:10.1101/pdb.rec11891 Cold Spring Harb Protoc 2009: pdb.rec11891-
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