Showtime solution
| Reagent | Amount to add | Final concentration |
|---|---|---|
| Tris (1 M; pH 8.2 or 9.5)a | 5 mL | 100 mM |
| MgCl2 (1 M) | 2.5 mL | 50 mM |
| NaCl (5 M) | 1 mL | 100 mM |
| Tween 20 (20%) | 250 μL | 0.1% |
| H2O | to 50 mL | |
| aDifferent chromogenic substrates require showtime solutions of different pH. | ||
