Recipe

MMR (10X)

Reagent Amount to add
5 M NaCl 400 mL
1 M KCl 40 mL
1 M MgCl2 20 mL
1 M CaCl2 40 mL
0.5 M EDTA 4 mL
1 M HEPES 100 mL
H2O to 1.5 L
Adjust pH to 7.8 using 10 M KOH. Adjust final volume to 2 L with H2O and autoclave.
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  1. doi:10.1101/pdb.rec11906 Cold Spring Harb Protoc 2009: pdb.rec11906-
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