Recipe

PNA probe cocktail

Formamide (70% [v/v])

KCl (5 mM)

MgCl2 (1 mM)

Peptide nucleic acid (PNA) probe, Cy3- or FITC-conjugated, specific for the sequence of interest (0.2 μg/mL; e.g., Dako)

Tris-Cl (12 mM, pH 7.2)

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  1. doi:10.1101/pdb.rec11912 Cold Spring Harb Protoc 2009: pdb.rec11912-
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