Recipe

Microbial lysis buffer

EDTA (0.2 M, pH 8.0)

NaCl (50 mM)

Sarkosyl (1%, w/v)

Sodium deoxycholate (1%, w/v)

Tris-Cl (10 mM, pH 8.0)

Autoclave. Immediately before use, add:

Lysozyme (1 mg/mL)

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  1. doi:10.1101/pdb.rec11918 Cold Spring Harb Protoc 2009: pdb.rec11918-
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