Recipe

FVM

Reagent Amount to add
6,7-dimethyl-5,6,7,8-tetrahydropterine hydrochloride (Sigma D0387) 1.5 g
L-Ascorbic acid (Calbiochem 1831) 15 mg
Glutathione (Calbiochem 3541) 75 mg
H2O 300 mL
Adjust the pH to 5.5. Filter through a 0.22-μm filter. Divide into 60 aliquots of 5 mL each. Store frozen.
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  1. doi:10.1101/pdb.rec11921 Cold Spring Harb Protoc 2009: pdb.rec11921-

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