Recipe

MEM + B-27

Reagent Amount to add
B-27 supplement, serum-free (50X; Invitrogen 17504044) 4 mL
D-(+)-glucose, SigmaUltra (200 mg/mL; Sigma G7528) 8 mL
MEM vitamin solution (100X; Invitrogen 11120052) 2 mL
Minimum essential medium (MEM; Invitrogen 11090081) 185 mL
Penicillin-streptomycin (Invitrogen 15140122) (optional) 1 mL
Filter through a 0.22-μm filter.
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  1. doi:10.1101/pdb.rec11923 Cold Spring Harb Protoc 2009: pdb.rec11923-
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