Table

Table 1. Chromagens used for in situ hybridization

Substrates Final conc. (μg/mL) Staining buffers Color Notes
BCIP/NBT in showtime solution 175/337.5 Showtime solution (pH 9.5) Purple Works very well. Most sensitive.
Magenta-phos/INT 250/250 Showtime solution (pH 9.5) Orange 9 h for full color development. Not time stable.
FastRed/NABP 250/500 Showtime solution (pH 8.2) Red About 6 h for full color development. Alcohol soluble.
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  1. doi:10.1101/pdb.tab195262 Cold Spring Harb Protoc 2009: pdb.tab195262-
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