Recipe

Lysis buffer (for amphioxus)

Reagent Quantity (for 50 mL) Final concentration
Tris-Cl (1 M; pH 8) 0.5 mL 10 mM
EDTA (0.5 M; pH 8) 10 mL 100 mM
SDS (sodium dodecyl sulfate) (10%) 2.5 mL 0.5% (w/v)
Mix the reagents and add H2O to give a final volume of 50 mL. Store at room temperature.
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  1. doi:10.1101/pdb.rec11927 Cold Spring Harb Protoc 2009: pdb.rec11927-
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