Recipe

Hybridization buffer (A)

Reagent Quantity (for 50 mL) Final concentration
Formamide (deionized) 25 mL 50% (v/v)
SSC (20X; pH 7) 12.5 mL 5X
Yeast RNA (10 mg/mL) 5 mL 1 mg/mL
Heparin 5 mg 100 μg/mL
Denhardt’s reagent (100X) 0.5 mL 1X
Tween 20 (10%) 0.5 mL 0.1% (v/v)
EDTA (0.5 M; pH 8) 0.5 mL 5 mM
Mix the reagents and add diethyl pyrocarbonate (DEPC)-treated H2O to give a final volume of 50 mL. Store at −20°C.
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  1. doi:10.1101/pdb.rec11932 Cold Spring Harb Protoc 2009: pdb.rec11932-
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