Recipe

Modified Barth’s saline (MBS) (1X, pH 7.8)

Reagent Quantity (for 1 L) Final concentration (1X)
NaCl 5.143 g 88 mM
KCl 0.075 g 1 mM
MgSO4 0.120 g 1 mM
HEPES 1.192 g 5 mM
NaHCO3 0.210 g 2.5 mM
CaCl2, dihydrate 0.103 g 0.7 mM
H2O to 1 L
Adjust the pH to 7.8 with 10 M NaOH and sterilize by autoclaving. Store at room temperature indefinitely.
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  1. doi:10.1101/pdb.rec11946 Cold Spring Harb Protoc 2009: pdb.rec11946-
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