Recipe

DEPC-H2O

Mix 1 mL of DEPC (diethyl pyrocarbonate) with 1000 mL of water in a screw-cap glass bottle. Incubate for ~2 h at room temperature in a fume hood with occasional swirling. Autoclave. Store at room temperature for up to 12 mo.

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  1. doi:10.1101/pdb.rec12068 Cold Spring Harb Protoc 2010: pdb.rec12068-

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