Recipe

Rabbit embryo culture medium (RECM)

Ham’s F10 medium with Phenol Red, NaHCO3, and stable glutamine (e.g., Biochrom FG0715)

Penicillin (50 U/mL)

Streptomycin (50 μg/mL)

Fetal calf serum (FCS; 20%) (optional, as per the requirements of the individual experiment)

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  1. doi:10.1101/pdb.rec12070 Cold Spring Harb Protoc 2010: pdb.rec12070-
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