Feeding bacteria
MATERIALS
Reagents
Glycerol, sterile
Klebsiella pneumoniae, wild-type, avirulent, ampicillin-resistant
Although this is the standard feeding bacterial strain, other enterobacteria can be used.
Equipment
Freezer preset to -80°C
Incubator preset to 37°C
Inoculation loop
Refrigerator preset to 4°C
Tubes, microcentrifuge, 2-mL
METHOD
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1. Grow the bacterial culture overnight in LB liquid medium.
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2. Distribute 0.5-mL aliquots of the culture into 2-mL tubes containing 0.5 mL glycerol.
Store tubes at -80°C.
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3. Each week, thaw one tube. Streak thawed culture onto one (or more) LB-ampicillin plates. Incubate overnight at 37°C.
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4. The following day, streak each of seven tubes with one colony from the Petri dish. Incubate overnight at 37°C.
Store cultures at 4°C for use the following day.
