Recipe

Wheat grass medium

Reagent Quantity (for 20 L)
Rich medium Standard medium
Na2HPO4•12H2O (50 mM) 1 L 1 L
Wheat grass stock solution 1 L 200 mL
Water, Millipore-filtered to 20 L to 20 L
Autoclave for 20 min at 120°C.
For rich medium, only use wheat grass stock solution prepared with wheat grass powder.
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  1. doi:10.1101/pdb.rec12079 Cold Spring Harb Protoc 2010: pdb.rec12079-
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