Recipe

Gelatin/BSA embedding medium

Reagent Amount to add (for 500 mL) Final concentration (w/v)
Gelatin 2.2 g 0.4%
Bovine serum albumin (BSA) 135 g 27%
Sucrose 90 g 18%
DEPC-PBS (10X), diluted to 1X to 500 mL --
Add the gelatin, BSA, and the sucrose to 450 mL of DEPC-PBS, stirring constantly until all ingredients are dissolved. Adjust the volume to 500 mL. Store 50-mL aliquots for up to 12 mo at -20°C. Store thawed aliquots refrigerated at 4°C; use within 1 wk.
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  1. doi:10.1101/pdb.rec12086 Cold Spring Harb Protoc 2010: pdb.rec12086-
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