Plastic culture rings
MATERIALS
Equipment
Box, sterile
Pipettes, transfer, plastic, 3.5-mL
Laboratory tissues
Razor blade
Vibratome
METHOD
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1. Use a razor blade to excise a region from a plastic transfer pipette at which the inner diameter is 3-5 mm.
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2. Embed the pipette piece in gelatin/BSA embedding medium.
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3. Cut the pipette piece into 300-μm-thick rings using the vibratome at very low speed.
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4. Sterilize the rings by washing in 70% ethanol.
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5. Wash the rings thoroughly in sterile water. Dry on tissues.
Store rings in a sterile box until use.
